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Boster Bio
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Siemens AG
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CarThera Inc
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Millar Inc
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Ultran Laboratories Inc
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InSightec inc
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Gallus BioPharmaceuticals
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Image Search Results
Journal: European journal of immunology
Article Title: Influenza A virus abrogates IFN-gamma response in respiratory epithelial cells by disruption of the Jak/Stat pathway.
doi: 10.1002/eji.200737045
Figure Lengend Snippet: Figure 11. Detection of IFNGR1, IFNGR2, Jak1, Jak2, and Stat1a expression in influenza A virus-infected A549 cells. A549 cells were infected with influenza Aichi at an MOI of 10 or left uninfected as described in Fig. 2. The infected cells and uninfected control cells were treated with IFN-c (1000 U/mL) for 1 h or left untreated. (A) Total RNA was extracted and subject to RT-PCR analysis with primers specific for IFNGR1, IFNGR2, Jak1, Jak2, and Stat1. (B) The protein levels were detected by western blot assay. A549 cells were infected and treated with IFN-c as described above. Total cell lysates (40 lg) were directly analyzed by western blotting with Ab to IFNGR1, IFNGR2, Jak1, Jak2, and Stat1a. Results shown are representative of three separate experiments.
Article Snippet: For detection of IFNGR1, IFNGR2, Jak1, and Jak2 , primary Ab used for western analyses included a rabbit polyclonal affinity purified anti-IFNGR1 Ab (1:2000; Santa Cruz Biotechnology), a
Techniques: Expressing, Virus, Infection, Control, Reverse Transcription Polymerase Chain Reaction, Western Blot